HAA-based Ion Pair Reversed Phase HPLC Method for More Accurate gRNA Purity Test

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• This document titled "HAA-based Ion Pair Reversed Phase HPLC Method for More Accurate gRNA Purity Test," is derived from the poster presented at ASGCT in May 2024. It highlights the development of an RP-HPLC method tailored specifically for CRISPR gRNAs. Although capillary electrophoresis is often employed for DNA and RNA quality assessments, our findings indicate that it does not provide the resolution necessary for accurate gRNA quality evaluation. The optimized RP-HPLC method we present will contribute significantly to the better understanding of gRNA safety.